Specialty
BAC Water
- Size
- 10ml
Specifications
BAC Water Technical Profile
OVERVIEW
What Is Bacteriostatic Water?
Bacteriostatic water (commonly referred to as BAC water) is sterile water that contains 0.9% benzyl alcohol as a bacteriostatic preservative. Unlike plain sterile water, which must be used immediately after opening due to contamination risk, bacteriostatic water is designed for multi-dose use, the benzyl alcohol inhibits microbial growth, allowing the vial to be accessed multiple times over a 28-day period while maintaining sterility.
BAC water for peptides is the preferred reconstitution solvent in research laboratories that work with lyophilized peptides. Bacteriostatic water 30ml vials from VivePeptides are manufactured to USP grade standards and USA tested to ensure consistent quality for peptide reconstitution. The 0.9% benzyl alcohol concentration provides effective bacteriostatic activity without interfering with peptide stability or activity in most research applications. When researchers buy bacteriostatic water from a trusted source like VivePeptides, they ensure their reconstituted peptides maintain integrity throughout multi-use research protocols.
- 01
Bacteriostatic Solvent
Sterile water with 0.9% benzyl alcohol for multi-use peptide reconstitution
- 02
USP Grade
Manufactured to United States Pharmacopeia standards
- 03
USA Tested & Verified
Quality tested at USA-based facilities to confirm sterility and composition
RESEARCH
Bacteriostatic Water Mechanism of Action in Research
The mechanism by which bacteriostatic water maintains sterility during multi-use applications centers on the bacteriostatic properties of benzyl alcohol. Understanding this mechanism helps researchers make informed decisions about solvent selection for their peptide research.
Benzyl Alcohol Bacteriostatic Activity
Benzyl alcohol at 0.9% concentration inhibits microbial growth through disruption of bacterial cell membrane integrity. The alcohol permeabilizes bacterial membranes, disrupting the proton motive force and interfering with energy-dependent cellular processes. This mechanism is bacteriostatic (growth-inhibiting) rather than bactericidal (killing), meaning it prevents new microbial growth from contaminating the solution during repeated access.
Peptide Compatibility
Research has demonstrated that 0.9% benzyl alcohol is compatible with the vast majority of research peptides. The low concentration does not significantly alter solution pH, ionic strength, or peptide folding in most applications. Studies indicate that reconstituted peptides in bacteriostatic water maintain stability and activity profiles comparable to those reconstituted in plain sterile water, with the added benefit of extended multi-use capability.
Multi-Use Sterility Maintenance
The practical advantage of bacteriostatic water in research settings is its ability to maintain a sterile field across multiple withdrawals. Each time a needle penetrates the rubber stopper, trace microorganisms may be introduced. The benzyl alcohol prevents these organisms from proliferating, maintaining solution sterility for up to 28 days when proper aseptic technique is used. ---
COMPARISON
Bacteriostatic Water vs Sterile Water: Research Solvent Comparison
Bacteriostatic Water
Understanding the difference between bacteriostatic water vs sterile water is essential for proper peptide reconstitution in research settings.
Sterile Water
For most peptide research applications, bacteriostatic water is the preferred choice due to its multi-use capability and extended post-opening stability. Sterile water is appropriate when benzyl alcohol must be avoided or when the entire vial will be used in a single session. Buy bacteriostatic water from VivePeptides for USP grade quality from a trusted USA supplier.
| Feature | Bacteriostatic Water | Sterile Water |
|---|---|---|
| Preservative | 0.9% Benzyl Alcohol | None |
| Multi-Use | Yes, up to 28 days after opening | No, single use only |
| Sterility After Opening | Maintained by bacteriostatic agent | Compromised upon first access |
| Peptide Compatibility | Compatible with most research peptides | Compatible with all peptides |
| Shelf Life (Opened) | 28 days | Immediate use required |
| USP Grade Available | Yes | Yes |
| Cost per Reconstitution | Lower (multi-use) | Higher (single-use waste) |
| Best For | Multi-use research peptide reconstitution | Single-use applications, benzyl alcohol-sensitive compounds |
RESEARCH STUDIES
Bacteriostatic Water Research Applications & Published Studies
Bacteriostatic water is a foundational research reagent with documented applications across pharmaceutical science, microbiology, and peptide chemistry.
Benzyl Alcohol Preservative Efficacy
Published research on benzyl alcohol as a pharmaceutical preservative spans decades. The United States Pharmacopeia (USP) has established monographs defining the specifications for bacteriostatic water, including benzyl alcohol concentration, sterility testing requirements, and endotoxin limits. Studies have validated the antimicrobial efficacy of 0.9% benzyl alcohol against common environmental contaminants under standard laboratory conditions.
Peptide Reconstitution and Stability Studies
Published research in pharmaceutical sciences has examined the stability of various peptides reconstituted in bacteriostatic water. Studies indicate that benzyl alcohol at the standard 0.9% concentration does not significantly affect peptide secondary structure, biological activity, or degradation kinetics for most research peptides. Manning et al. (Pharmaceutical Research, 2010) published comprehensive reviews on peptide stability in various solvent systems relevant to research applications.
Laboratory Best Practices
Research on aseptic technique and reagent handling has established best practices for bacteriostatic water use in laboratory settings. Studies confirm that proper technique, including swabbing the stopper with 70% alcohol before each access and using sterile needles, maximizes the effective multi-use lifespan of bacteriostatic water in research environments.
QUALITY ASSURANCE
Quality & Testing Standards
HPLC & Mass Spectrometry
Every batch of bacteriostatic water undergoes sterility testing and endotoxin screening to confirm the absence of microbial contamination and pyrogens.
Third-Party Verified
All bacteriostatic water is independently verified through third-party USA-based testing facilities with lot-specific documentation.
≥99% Purity Standard
VivePeptides bacteriostatic water is manufactured to USP grade specifications, including verified 0.9% benzyl alcohol concentration and sterility.
FAQ
Frequently Asked Questions About Bacteriostatic Water
What is bacteriostatic water used for in research?
What grade is VivePeptides bacteriostatic water?
How should bacteriostatic water be stored?
Can bacteriostatic water be used with all peptides?
Is VivePeptides bacteriostatic water third-party tested?
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