Growth & Repair
IGF-1LR3
- Size
- 1mg
Specifications
IGF-1LR3 Technical Profile
OVERVIEW
What Is IGF-1 LR3 Peptide?
IGF-1 LR3 (Insulin-like Growth Factor 1 Long R3) is an 83-amino-acid synthetic variant of human IGF-1, engineered with two key structural modifications: a glutamic acid substitution for arginine at position 3 (R3) and a 13-amino-acid N-terminal extension peptide. These modifications were designed to reduce binding affinity for IGF binding proteins (IGFBPs), which normally sequester circulating IGF-1 and limit its bioavailability. As a result, IGF-1 LR3 exhibits a significantly extended functional half-life compared to native IGF-1 in research systems.
IGF-1 LR3 peptide is one of the most widely used growth factor analogs in cell biology and tissue culture research. Its reduced IGFBP binding means that a greater proportion of the peptide remains free to interact with the IGF-1 receptor (IGF-1R), making it a more potent and predictable tool for in vitro research. Published studies utilizing IGF-1 LR3 span cell proliferation, differentiation, survival signaling, and metabolic research, reflecting the peptide's broad utility in growth factor biology.
- 01
Modified IGF-1 Analog
83-amino-acid variant with reduced IGFBP binding and extended functional half-life
- 02
≥99% HPLC Purity
Every batch verified via high-performance liquid chromatography
- 03
USA Tested & Verified
Third-party analytical testing performed in USA laboratories
RESEARCH
IGF-1 LR3 Mechanism of Action in Research
The IGF-1 LR3 mechanism of action parallels that of native IGF-1 but with enhanced potency due to its reduced IGFBP sequestration. Published research has extensively characterized IGF-1 LR3's interaction with the IGF-1 receptor and its downstream signaling cascades. IGF-1 LR3 research provides valuable data on growth factor signaling in contexts where extended receptor activation is experimentally desirable.
IGF-1 Receptor (IGF-1R) Activation
IGF-1 LR3 binds to and activates the IGF-1 receptor (IGF-1R), a receptor tyrosine kinase expressed on virtually all mammalian cell types. Upon binding, IGF-1R undergoes autophosphorylation, recruiting insulin receptor substrate (IRS) proteins and Shc adaptor molecules. Research demonstrates that IGF-1 LR3 activates the same downstream pathways as native IGF-1, primarily the PI3K/Akt/mTOR and Ras/MAPK/ERK cascades, but with greater potency due to its prolonged receptor availability. Francis et al. (1992) characterized the binding kinetics of Long R3 IGF-1, establishing its reduced IGFBP affinity profile.
PI3K/Akt/mTOR Signaling Cascade
A primary downstream pathway of IGF-1R activation is the PI3K/Akt/mTOR cascade, which regulates cell survival, protein synthesis, and metabolic homeostasis. Research indicates that IGF-1 LR3's sustained receptor activation leads to prolonged Akt phosphorylation and enhanced mTOR-mediated translation initiation in cell culture systems. Studies by Coolican et al. (1997) demonstrated that IGF-1-mediated PI3K/Akt signaling plays essential roles in myoblast differentiation models, with IGF-1 LR3 serving as the preferred growth factor due to its predictable dose-response characteristics.
MAPK/ERK Proliferation Pathway
IGF-1 LR3 also activates the mitogen-activated protein kinase (MAPK/ERK) signaling pathway through Ras-Raf-MEK-ERK activation. This pathway drives cell cycle progression and proliferation responses. Research by Duan et al. (2010) utilized IGF-1 and its analogs to investigate the relative contributions of PI3K/Akt versus MAPK/ERK signaling in IGF-1R-mediated cellular responses, demonstrating context-dependent pathway utilization across different cell types. ---
COMPARISON
IGF-1 LR3 vs Standard IGF-1: Research Peptide Comparison
IGF-1 LR3
IGF-1 LR3 and standard (native) IGF-1 share the same receptor target but differ significantly in their pharmacokinetic profiles and research utility. Understanding these differences is essential for researchers selecting the appropriate growth factor for their experimental systems.
Standard IGF-1
IGF-1 LR3's reduced IGFBP binding makes it the preferred choice for in vitro research where consistent, predictable growth factor exposure is required. Standard IGF-1 remains important for in vivo studies where IGFBP interactions are part of the biological system under investigation. VivePeptides offers research grade IGF-1 LR3 for sale at ≥99% purity from our USA-based facility.
| Feature | IGF-1 LR3 | Standard IGF-1 |
|---|---|---|
| Amino Acids | 83 | 70 |
| Molecular Weight | 9117.5 Da | 7649 Da |
| Key Modification | R3E substitution + 13-aa N-terminal extension | Native human sequence |
| IGFBP Binding | Dramatically reduced | High affinity for 6 IGFBPs |
| Effective Half-Life | Extended (20-30 hours in vitro) | Short (~10 min in serum) |
| Relative Potency | 2-3× more potent in cell culture | Baseline reference |
| Primary Research Use | Cell culture, in vitro growth factor studies | In vivo models, binding protein studies |
| Cost Efficiency | Higher due to extended activity | Lower per µg, but shorter duration |
| Research Volume | Widely used in cell culture protocols | Gold standard in vivo |
RESEARCH STUDIES
IGF-1 LR3 Research Applications & Published Studies
IGF-1 LR3 research is deeply integrated into cell biology, tissue engineering, and growth factor signaling literature. The following areas represent the most actively investigated applications. All references are to research contexts only.
Cell Proliferation and Culture Media Research
IGF-1 LR3 is a standard component in serum-free and reduced-serum cell culture media formulations. Francis et al. (1992) published foundational work in the Journal of Molecular Endocrinology characterizing Long R3 IGF-1's enhanced potency in cell proliferation assays compared to native IGF-1. The peptide's predictable dose-response and extended stability in culture media have made it a preferred growth factor supplement in CHO cell culture, stem cell expansion, and primary cell isolation protocols across hundreds of published studies.
Myogenesis and Muscle Biology Research
Research has extensively used IGF-1 LR3 in myoblast differentiation and muscle biology studies. Coolican et al. (1997) published findings in the Journal of Biological Chemistry demonstrating that IGF-1-mediated signaling through PI3K is essential for myoblast differentiation. Subsequent studies by Rommel et al. (2001) used IGF-1 and its analogs to map the Akt/mTOR pathway's role in muscle hypertrophy models, with IGF-1 LR3 providing sustained pathway activation in cell culture experiments.
Stem Cell and Tissue Engineering Applications
IGF-1 LR3 has been incorporated into numerous stem cell research and tissue engineering protocols. Published studies by Blum & Bhatt (2003) and others have utilized IGF-1 LR3 as a critical component in defined media for embryonic stem cell and induced pluripotent stem cell (iPSC) maintenance. The peptide's role in promoting cell survival and proliferation without batch-to-batch variation of serum makes it invaluable in reproducible stem cell research protocols.
QUALITY ASSURANCE
Quality & Testing Standards
VivePeptides maintains rigorous quality control for every batch of IGF-1 LR3 peptide. Our commitment to research-grade purity ensures that laboratories receive consistent, reliable compounds for their investigations.
HPLC & Mass Spectrometry
Every batch of IGF-1 LR3 undergoes high-performance liquid chromatography (HPLC) and mass spectrometry analysis to confirm identity, purity, and molecular weight.
Third-Party Verified
All VivePeptides IGF-1 LR3 is independently verified through third-party analytical laboratories based in the USA. Testing documentation is available for every lot.
≥99% Purity Standard
Our IGF-1 LR3 consistently meets or exceeds ≥99% purity as determined by HPLC analysis, ensuring research-grade quality for laboratory applications.
FAQ
Frequently Asked Questions About IGF-1 LR3
What is IGF-1 LR3 used for in research?
What purity is VivePeptides IGF-1 LR3?
How should IGF-1 LR3 be stored?
What is the difference between IGF-1 LR3 and standard IGF-1?
Is VivePeptides IGF-1 LR3 third-party tested?
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IGF-1LR3
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